The innate mechanisms

Constitutive immune mechanisms: mediators of host defence and immune regulation - Constitutive and inducible mechanisms

슬로싱킹 2024. 10. 6. 19:01

 

41577_2020_Article_391.pdf
2.66MB

 

A major challenge for living organisms is to maintain homeostasis in response to changes in external and internal environments. These include alterations in nutrient and water supplies, physical stress, temperature changes, physiological stress, infections and malignancies.

 

 homeostasis 항상성

 alteration 변화

malignancy 악성종양

 

Through billions of years of evolution, the forms of life and biological processes that cope with these challenges in the most successful way have been selected. One challenge that all organisms have to deal with is the elimination of microorganisms and of abnormal or damaged cellular material.

cope with ~에 대처하다

 elimination 제거하다

abnormal 비정상적인

 

The ideal immune response would eliminate the potential threat and re-establish homeostasis without causing excessive damage to healthy cells and tissues. However, immune responses to infections are often disruptive and can cause marked tissue damage.

 

 eliminate 없애다

re-establish ~을 재건하다

homeostasis 항상성

 excessive 지나친, 과도한

disruptive 지장을 주는

 

Such responses are evolutionarily advantageous when the benefit of eliminating the challenge outweighs the risk of associated tissue damage and the requirement for regeneration. However, for potential challenges that occur frequently but rarely develop into serious homeostasis-altering threats, it is not desirable to mount systemic or potentially disruptive immune responses.

 

 evolutionarily 진화론적으로

advantageous 이로운

 outweigh ~보다 더 크다

regeneration 재생

desirable 바람직한

systemic 전체에 영향을 주는

 

In addition, vigorous immune responses are not desirable in organs and tissues that are particularly sensitive to immune-mediated damage, such as the brain. Therefore, the ideal immune response has checks and balances, which allow the organism to modulate the magnitude and duration of the response according to the nature of the threat caused by the challenge.

 

vigorous 격렬한

desirable 바람직한

particularly 특히

modulate 조절하다

magnitude 규모

duration 지속

 

The mammalian immune system, as we understand it today, is induced mainly by two types of receptor systems, the germline-encoded pattern recognition receptors (PRRs), which initiate innate immune responses, and the antigen-specific receptors generated through gene rearrangement after antigen encounter, which initiate adaptive immune responses.

 

rearrangement 재배열

encounter 맞닥드리다

 

The immune responses induced by PRRs, such as Toll-like receptors (TLRs), interact with those induced by antigen-specific receptors; this interaction is notably represented by dendritic cells, which rely on PRR-driven cues to initiate dendritic cell maturation for the stimulation of lymphocytes through antigen-specific receptors.

 

 notably 특히

cue 신호

stimulation 자극

initiate 개시되게하다

 

However, the research literature contains numerous reports of host defence activities that occur independently of both PRR-based immunity and antigen-specific receptors, and emerging evidence suggests that several of these mechanisms have non-redundant roles in host defence in humans.

 

literature 문학

independently of ~와 관계없이

non-redundant 중복되지 않는

 

Here we review the literature on this topic by focusing on constitutive immune mechanisms. On the basis of this analysis, and by integrating concepts previously reviewed, we propose that this constitutive layer of innate immunity exerts early host defence activities through specific molecular mechanisms and at the same time limits PRR activation as a specific feature.

 

constitutive 구성요소인

integrating 통합시키다

exert (영향력을) 가하다

 

 

Constitutive and inducible mechanisms

The innate immune system uses both constitutive and inducible mechanisms to eliminate infections and damaged self to maintain homeostasis.

 

eliminate 없애다

fig.1 Constitutive innate immune responses versus inducible immune responses

 

Although the constitutive mechanisms have the advantage of providing an immediate response to a danger signal, they lack the potential to amplify the response. In addition, constitutive mechanisms consume energy to remain operative, and there are hence limits to how many of these can be maintained in any one organism.

 

immediate 즉각적인

amplify 증폭시키다

consume 소모하다

hence 이런이유로

 

By contrast, inducible mechanisms such as those mediated through PRRs, as well as antigen-specific receptors, are activated only in response to stimuli and have the ability to amplify signals many times. Hence, inducible mechanisms can give rise to very strong and efficient immune responses, but can also lead to excess inflammation and immunopathology.

 

By contrast 대조적으로

mediate 중재하다

 stimulus(stimuli) 자극제(의 복수형)

amplify 증폭시키다

 excess 지나침

immunopathology 면역병리학

 

Given their amplification potential, inducible immune mechanisms require tight control and negative regulatory systems. The constitutive immune mechanisms can be divided into the chemical and physical barriers of the body, such as skin, saliva, stomach acid and urine flow, which are not the focus of this Review, and various molecularly defined mechanisms that control microbial infection and/or replication.

 

amplification 증폭

potential 잠재력

inducible 유도성의, 유발성의

 tight 단단한

regulatory 규제력을 지닌

saliva 침, 타액

 replication 응답

 

Although these mechanisms have been known for many years, they have generally been considered to have only minor roles in the immune system, and evidence has been lacking as to their specific, non-redundant functions in host defence.

 

Consequently, they have not received much attention in front-line immunology research. Here we discuss the constitutive innate immune responses in comparison with the better-described inducible innate responses triggered by PRRs. In addition, we present evidence suggesting that efficient action of constitutive innate immune mechanisms leads to both antimicrobial activity and mitigation of PRR-driven activities (Fig. 2).

 

comparison 비교

 inducible 유치할 수 있는

mitigation 완화

 

fig.2 Constitutive innate immune responses negatively regulate inducible immune responses

PRR-activated inducible innate immune responses.

PRRs detect pathogen-associated molecular patterns (PAMPs), microorganism-associated molecular patterns, host derived danger-associated molecular patterns and molecular signatures associated with homeostasis altering molecular processes.

 

detect 감지하다

derived 파생된

 

These molecular patterns activate PRR signalling, which ultimately leads to the transcription of antimicrobial and proinflammatory genes. Downstream activities of PRR signalling include the production of type I interferon (interferon-α (IFNα) and IFNβ), IL-1β and tumour necrosis factor (TNF).

 

 ultimately 궁극적으로

 transcription 표기

proinflammatory 염증전

 

These cytokines, in turn, activate antimicrobial and proinflammatory activities, as well as the maturation of antigen-specific adaptive immune responses. PRR-based immune responses can be highly potent, and numerous inflammatory diseases are driven by excessive PRR signalling pathways.

 

However, the nature of PRR-based immunity is influenced by many factors, and it is worth mentioning that the gut microbiota and chronic viral infections can induce PRR-based, host-beneficial responses that tend towards tolerance rather than inflammation.

 

gut microbiota 장내 미생물군

chronic viral 만성 바이러스

tolerance 내성

 

Nevertheless, given the potency of PRR-based immunity, full activation of PRR-driven immune responses each time a microorganism is encountered may not be beneficial for an organism in the longer term. Moreover, it is essential to control the activation and duration of PRR signalling-induced activities.

 

 potency 힘

activation 활성화

each time 언제나

encounter 맞딱드리다

duration 지속

 

This is achieved through multiple mechanisms, including two-step procedures for full PRR activation, the requirement for a threshold PAMP concentration to achieve PRR activation, amplification loops from initial low responses and numerous negative-feedback mechanisms.

 

procedures 절차

threshold concentration 최저 유효 농도

amplification 증폭

 

One way in which the activation of PRR signalling in response to very low levels of PAMPs is avoided at the molecular level is through supramolecular organizing centres. These are higher-order signalling complexes at specific subcellular locations that rely on amplification mechanisms to achieve full activation, thus preventing signalling by subthreshold levels of PAMPs but amplifying signalling by superthreshold levels of PAMPs.

 

activation 활성화

 

supramolecular organizing centres(SMOCs)

kagan2014.pdf
1.19MB

 

higher-order 고차원

subthreshold (자극이) 반응을 일으키기에는 불충분한

 

 

The double-edged sword-like nature of PRR-induced immune responses in terms of their roles in both protection and disease is also supported by evolutionary evidence. This includes the recurring loss of 2′-5′-oligoadenylate synthase 1 (OAS1) in primates. OAS1 is an interferon-inducible protein that is associated with both antiviral and pathological activities.

 

double-edged sword (긍정과 부정의) 양면성을 지닌 상황[결정]

 

 

Constitutive innate immune mechanisms.

Constitutive innate immune mechanisms respond to microbial activities, cellular stress and metabolic alterations by inducing antimicrobial effector functions. As there is most evidence for constitutive innate immune mechanisms that exert antiviral and antibacterial activities, these are the focus of this Review.

 

metabolic 신진대사의

alterations 변화

 constitutive 구성요소인

exert 가하다

 


fig.3 Overview of the regulation of microbial replication by constitutive innate immune mechanisms.

 

 

a | Constitutive innate immune mechanisms and viral infection. The accumulation of specific viral molecular structures (such as double-stranded RNA (dsRNA) or capsids) and cellular stress responses (such as autophagy) activate constitutive–latent mechanisms with direct antiviral activity, independently of pattern recognition receptors.

 

accumulation 늘어나다

constitutive 구성요소인

latent 잠재하는

independently of ~와 관계없이

 

Some of the antiviral effector functions target microbial replication by blocking specific steps in the replication cycles of viruses; these effectors include soluble lectins, antimicrobial peptides, restriction factors, RNA interference (RNAi) and metabolites. Other antiviral effectors of the constitutive response function through the degradation of virus particles; these include nucleases such as TREX1, which degrades viral DNA in the cytoplasm, and RNase L, which degrades viral RNA, as well as autophagy and proteasomal degradation. Viruses can be targeted for proteasomal degradation by the ubiquitin E3 ligase TRIM21, which binds to antibody-attached viral capsids.

 

effector 반응기

 replication 복제

metabolites 대사물질

constitutive 구성요소인

degradation 분해

 

 

 

 b | Constitutive innate immune mechanisms and bacterial infection. The presence of bacteria changes the local microenvironment, for example through the accumulation of hydrophobic and charged bacterial surfaces or alteration of cellular metabolism. This activates antibacterial activities independently of pattern recognition receptors, including inactivation by soluble lectins and antimicrobial peptides, nutritional depletion by natural resistance-associated macrophage protein 1 (NRAMP1) and lactoferrin, and bacterial degradation by phagocytosis and basal autophagy. dsDNA, double-stranded DNA; RISC, RNA-induced silencing complex; ROS, reactive oxygen species; viRNA, virus-derived small interfering RNA.

 


 

A large range of constitutive mechanisms of innate immunity have been identified, including restriction factors, antimicrobial peptides, basal autophagy and proteasomal degradation.

 

constitutive 구성요소인

identified 식별된

restriction 제한

degradation 분해

 

 

Here we divide these mechanisms into two classes: those that target specific steps in microbial replication cycles, such as restriction factors, and those that lead to degenerative processes, such as autophagy.

 

replication cycles 복제주기

 

The constitutive mechanisms that target specific steps in microbial replication function by blocking molecularly defined events that are essential for the replication of specific microorganisms but are dispensable for cellular fitness.

 

molecularly 분자에 의하여 

dispensable 불필요한

fitness (신체적인) 건강

 

 

By contrast, those mechanisms that operate through degenerative programmes target microbial or altered host molecules for recycling or degradation. The modes of action of representative examples from each of these mechanistic classes are described in the following sections.

 

By contrast 대조적으로

degenerative 퇴행성의

degradation 분해

mechanistic 기계론적인

 

 

Given the ability of constitutive immune mechanisms to exert antimicrobial activity, one consequence of their successful action is decreased levels of PAMPs (Fig. 2a). This, in turn, limits PRR activation and the downstream inflammatory response (Fig. 2b).

 

exert 가하다

Fig. 2 ❘ Constitutive innate immune responses negatively regulate inducible immune responses

 

 

Thus, constitutive immune mechanisms equip cells and tissues with a layer of defence that can fight infections immediately and hence potentially limit the requirement for inducible immune responses, such as type I interferon, IL-1β and other proinflammatory cytokines.

 

 equip 준비를 갖춰주다

potentially 잠재력

inducible 유치할 수 있는

 proinflammatory 염증전