DEEP WORK

DNA ISOLATION AND PURIFICATIONBasic to all biotechnology research is the ability to manipulate DNA. First and foremost for recombinant DNA work, researchers need a method to isolate DNA from different organisms. Isolating DNA from bacteria is the easiest procedure because bacterial cells have little structure beyond the cell wall and cell membrane. Bacteria such as E. coli are the preferred org..

THE CENTRAL DOGMA OF MOLECULAR BIOLOGYTwo essential features of living creatures are the ability to reproduce their own genome and manufacture their own energy. To accomplish these feats, an organism must be able to make proteins using information encoded in its DNA. Proteins are essential for cellular architecture, giving the cell a particular shape and structure. Proteins include enzymes that ..

ESCHERICHIA COLI IS THE MODEL BACTERIUMAlthough extreme bacteria are interesting and useful, more typical bacteria are the routine workhorses for research in molecular biology and biotechnology. The most widely used is Escherichia coli, a rod-shaped bacterium about 1 by 2.5 microns in size. E. coli normally inhabits the colon of mammals including humans (Fig. 1.6) FIGURE 1.6 Subcellular Structu..