METHODS OF DETECTION FOR NUCLEIC ACIDS Recombinant DNA methodologies require the ability to detect DNA. One of the easiest ways to detect the amount of DNA or RNA in solution is to measure the absorbance of ultraviolet light at 260 nm (Fig. 3.4). DNA absorbs ultraviolet light because of the ring structures in the bases. Single-stranded RNA and free nucleotides also absorb ultraviolet light. In f..
